Allergen Details:
| Allergen name: | Cup s 2 |
|---|---|
| Allergen source: |
Major taxonomic group: Plantae Pinopsida Order: Cupressales Species: Cupressus sempervirens (Common cypress) NCBI Taxomony ID: 13469 |
| Biochemical name: | Polygalacturonase |
| MW(SDS-PAGE): | 43 kDa |
| Allergenicity: | sera from 19 of 31 subjects with airway allergy in areas exposed to Common cypress (Curessus sempervirens) were positive to a 43 kDa protein based on immunoblots. The protein was characterized and demonstrated to be highly identical to Jun a 2 (~95% identity) and to Cha o 2 (80%). |
| Allergenicity reference: | PubMed:28439939 |
| Route of allergen exposure: | Airway |
| Date Created: | 29-05-2017 |
| Last Updated: | 2019-08-09 07:16:52 |
| Submitter Info: | |
| Name: | Pascal Ponset |
| Institution: | Institut Pasteur |
| City: | Paris, France |
| Email: | pascal.poncet@pasteur.fr |
| Submission Date: | 2016-12-09 |
Comments
Note that the Uniprot entry for the protein sequence shows a number of gaps between peptide segments, and BLAST to the nearest sequence suggests that there are many gaps. Thus the UniProt sequence C0HKB1 is likely to be wrong.
| Isoallergen and variants | GenBank Nucleotide | GenBank Protein | UniProt | PDB |
|---|---|---|---|---|
| Cup s 2.0101 | C0HKB1 |
Ten reads of LC-MSMS produced 33 overlapping peptides with high identity match to a homologous protein from Cupressus arizonica and to Juniperus ashei, with >74% coverage. Edmand degradation was used to identify the N-terminus. That sequence was submitted to UniProt as C0HKB1, which is not yet posted. Note the IgE binding to this protein in Allergy 2017 72(11):1806 shows total inhibition with bromelain. In addition, the sequence entered here and UniProt indicates many gaps as this was LC/MSMS