Allergen Details:
| Allergen name: | Bac s 1 |
|---|---|
| Allergen source: |
Major taxonomic group: Bacteria Firmicutes Order: Bacillales Species: Bacillus subtilis (Hay bacillus) |
| Biochemical name: | Nattokinase (subtilisin-like serine protease) |
| MW(SDS-PAGE): | 30 kDa |
| Allergenicity: | Eigth patients with IgE-mediated food allergy to natto (soybeans fermented with Bacillus subtilis subsp. natto) were tested. All patients had immediate allergic reactions after ingestion of natto as well as positive SPT with natto. Of these patients, 4/8 (50%) had IgE to a 30 kDa protein from natto as well as from an extract from cultured B. subtilis subsp. natto detected by IgE immunoblot. The protein was identified by immunoblot inhibition with commercially available nattokinase and by mass spectrometry. Moreover, all 4 sera reacted to nattokinase in ELISA. Three patients were also tested with nattokinase by SPT and showed a reaction to the protein. Purity of the purchased nattokinase was confirmed by mass spectrometry. |
| Allergenicity reference: | PubMed:36517353 |
| Route of allergen exposure: | Food |
| Date Created: | 2023-01-11 |
| Last Updated: | 2023-01-11 17:31:24 |
| Submitter Info: | |
| Name: | Masashi Nakamura |
| Institution: | Fujita Health University, Integrative Medical Science for Allergic Disease; Hoyu co. ltd, General Research and Development Institute |
| City: | Nagoya, Japan and Nagakute, Japan |
| Email: | manaka@fujita-hu.ac.jp, masashi_nakamura@hoyu.co.jp |
| Submission Date: | 2022-11-06 |
Comments
| Isoallergen and variants | GenBank Nucleotide | GenBank Protein | UniProt | PDB |
|---|---|---|---|---|
| Bac s 1.0101 | KY576911 | AUR45012 | A0A2I7MLQ7 | 5GL8 |